Due to their rare combination of high chemical stability, exceptional optical and electrical properties, high surface-to-volume ratio, and high aspect ratio, carbon nanotubes (CNTs) have made an enormous impact on materials science, molecular biology, biomedicine, and bioanalytical chemistry.
Carbon Nanotubes: Methods and Protocols provides reliable, consistent protocols on the application of CNTs in molecular biology-related fields. These are of vital importance, as the commercially available CNTs differ in purity, agglomeration state, as well as length and diameter distribution, all of which have a profound influence on the dispersability and surface properties of the tubes.
The volume contains detailed sections on functionalization, toxicity, trafficking, scaffolds, and biosensors, provided by expert researchers from various fields. Written in the highly successful Methods in Molecular Biology(TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Carbon Nanotubes: Methods and Protocols serves to contribute to the achievement of common standards and helps researchers to avoid discrepancies in future biology-related CNT studies.
From the reviews: "A comprehensive introduction to the application of carbon nanotubes (CNTs) in the life-sciences. ... It also provides detailed step-by-step procedures and complete lists of the reagents and materials required to carry out the applications introduced.
... 'Carbon Nanotubes: Methods and Protocols' is an indispensable tool for researchers looking for a comprehensive summary of the state-of-the-art of applications of CNTs. ... A valuable tool both for experts and for readers who intend to step into the fascinating world of CNT applications in biology and medicine."--- (Frank Schleifenbaum, Analytical and Bioanalytical Chemistry, Vol.
399, October, 2010)Spis treści:Part I: Functionalization 1. Non-Covalent Attachment of Proteins to Single-Walled Carbon Nanotubes Luis F. F. Neves, Ta-Wei Tsai, Naveen R. Palwai, David E. Martyn, Yongqiang Tan, David W.
Schmidtke, Daniel E. Resasco, and Roger G. Harrison 2. Covalent Conjugation of Multi-Walled Carbon Nanotubes with Proteins Changqing Yi, Suijian Qi, Dawei Zhang, and Mengsu Yang 3. Covalently Linked Deoxyribonucleic Acid with Multi-Walled Carbon Nanotubes: Synthesis and Characterization Weiwei Chen, Changqing Yi, Tzang Chi-Hung, Shuit-Tong Lee, and Mengsu Yang 4.
Temperature and pH-Responsive 'Smart' Carbon Nanotube Dispersions Dan Wang and Liwei Chen Part II: Toxicity 5. Effects of Carbon Nanotubes on the Proliferation and Differentiation of Primary Osteoblasts Dawei Zhang, Changqing Yi, Suijian Qi, Xinsheng Yao, and Mengsu Yang 6.
Carbon Nanotube Uptake and Toxicity in the Brain Leying Zhang, Darya Alizadeh, and Behnam Badie 7. In vitro and in vivo Biocompatibility Testing of Functionalized Carbon Nanotubes Gianni Ciofani, Vittoria Raffa, orazio Vittorio, Alfred Cuschieri, Tommaso Pizzorusso, Mario Costa, and Giuseppe Bardi 8.
Real-time Monitoring of Cellular Responses to Carbon Nanotubes Qingxin Mu, Shumei Zhai, and Bing Yan 9. Reducing Nanotube Cytotoxicity Using a Nano-Combinatorial Library Approach Qiu Zhang, Hongyu Zhou, and Bing Yan 10.
DNA Damage aby Carbon Nanotubes Using the Single Cell Gel Electrophoresis Technique Olga Zeni and Maria Rosaria Scarfi Part III: Trafficking 11. Assessment of Cellular Uptake and Cytotoxicity of Carbon Nanotubes Using Flow Cytometry Khuloud T.
Al-Jamal and Kostas Kostarelos 12. Cell Trafficking of Carbon Nanotubes Based on FluorescenceDetection Monica H. Lamm and Pu Chun Ke 13. Carbon Nanotubes as Intracellular Carriers for Multidrug Resistant Cells Studied żeby Capillary Electrophoresis-Laser Induced Fluorescence (CE-LIF) Ruibin Li, Hanfa Zou, Hua Xiao, and Renan Wu Part IV: Scaffolds 14.
Carbon Nanotube-Based Neurochips Moshe David-Pur, Mark Shein, and Yael Hanein 15. Effect of Carbon Nanotubes on HepG2 Adhesion and Spreading Suijian Qi, Changqing Yi, Dawei Zhang, and Mengsu Yang Part V: Biosensors 16.
Enzymatic Detection Based on Carbon Nanotubes Martin Pumera 17. Carbon Nanotube Biosensors Based on Electrochemical Detection Martin Pumera 18. Biosensors Based on Carbon Nanotube-Network Field-Effect Transistors Cristina C.
Cid, Jordi Riu, Alicia Maroto, and F. Xavier Rius 19. Detection of Biomarkers with Carbon Nanotube-Based Immunosensors Samuel Sanchez, Esteve Fabregas, and Martin Pumera 20. Carbon Nanotube Biosensors with Aptamers as Molecular Recognition Elements Hye-Mi So, Dong-Won Park, Hyunju Chang, and Jeong-O Lee